Antioxidative Effect of Phellinus Igniarius Polysaccharide on HaCaT Cells Based on Multi-parameter Analysis by AFM Imaging

Phellinus igniarius is widely used for anti-oxidation in China, and the effect of anti-oxidation of polysaccharides from Phellinus igniarius fruiting body (PIP) was studied in this article. AFM was used to scan the different groups of HaCaT cells. The HaCaT cells height, adhesion and Young's modulus were determined. In this study, the height of HaCaT cells were close gradually to the normal HaCaT cells after ethanol induced oxidative damage when cultured with PIP for 6-24 h, and the results were time-dependent. The adhesion of HaCaT cells cultred with PIP for 18h was closer to the normal cells than that with PIP for 12h. When the cells cultured with the PIP for 12-24 h, the HaCaT cells Young's modulus with ethanol induced oxidative damage was close to that of normal HaCaT cells. These results demonstrated that the effect of PIP against oxidative damage of HaCaT cells.