氧化应激
血管生成
炎症
细胞凋亡
刺激
医学
下调和上调
新生血管
血管内皮生长因子A
伤口愈合
移植
H&E染色
血管内皮生长因子
男科
糖尿病
免疫组织化学
内科学
免疫学
生物
内分泌学
生物化学
基因
血管内皮生长因子受体
作者
Cong Chen,Xiaolu Li,Yimin Hu,Yuan Chen,Hongrui Wang,Xian Li,Xiucun Li
出处
期刊:PeerJ
[PeerJ]
日期:2024-01-31
卷期号:12: e16856-e16856
摘要
Skin flap transplantation is one of the effective methods to treat the diabetes-related foot ulceration, but the intrinsic damage to vessels in diabetes mellitus (DM) leads to the necrosis of skin flaps. Therefore, the discovery of a non-invasive and effective approach for promoting the survival of flaps is of the utmost importance. Electrical stimulation (ES) promotes angiogenesis and increases the proliferation, migration, and elongation of endothelial cells, thus being a potential effective method to improve flap survival.The purpose of this study was to elucidate the mechanism used by ES to effectively restore the impaired function of endothelial cells caused by diabetes.A total of 79 adult male Sprague-Dawley rats were used in this study. Gene and protein expression was assessed by PCR and western blotting, respectively. Immunohistochemistry and hematoxylin-eosin staining were performed to evaluate the morphology and density of the microvessels in the flap.The optimal duration for preconditioning the flap with ES was 7 days. The flap survival area percentage and microvessels density in the DMES group were markedly increased compared to the DM group. VEGF, MMP2, and MMP9 protein expression was significantly upregulated. ROS intensity was significantly decreased and GSH concentration was increased. The expression of IL-1β, MCP‑1, cleaved caspase-3, and Bax were downregulated in the DMES group, while TGF-β expression was upregulated.ES improves the angiogenesis in diabetic ischemic skin flaps by attenuating oxidative stress-mediated inflammation and apoptosis, eventually increasing their viability.
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