核酸外切酶
素数(序理论)
配体(生物化学)
片段(逻辑)
化学
竞赛(生物学)
计算生物学
组合化学
计算机科学
生物
生物化学
DNA
程序设计语言
受体
数学
组合数学
DNA聚合酶
生态学
作者
Leonhard Geist,Paola Martinelli,Shereena Mohideen-Ali,Patrick Werni,Gerhard Fischer,Julian E. Fuchs,Klaus Rumpel,Moriz Mayer
标识
DOI:10.1016/j.jmro.2022.100075
摘要
Inhibition of the exonuclease activity of Three-prime Repair EXonuclease 1 (TREX1) has emerged as a potential novel immunotherapeutic strategy. Herein we describe our screening approach to find initial low-affinity fragment hits for TREX1 from our 19F library and the use of both ligand- and target-based NMR methods for hit confirmation and validation. In a further step to support the early hit expansion stage we describe our setup of a 19F competition assay for KI determination of analogs.
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