Uncovering the Functional Role of UGT73F/P Subfamilies toward Triterpenoids Glycosylation in Legume Medicinal Plants

, revealing that UGT73F/P enzymes mediate the primary glycosylation of triterpenoids with distinctive regioselectivity. This finding was further supported by a kingdom-wide mining effort and selective characterization among 286 UGT73F/P enzymes from 167 plant species, covering 54 families. In addition, we found that UGT73F and UGT73P enzymes were specific to legume species. Finally, we identified a K-region within the enzyme active site that governs the differentiated function of UGT73F/P enzymes. Our study highlights the great potential of UGT73F/P enzymes for precise triterpenoid glycosylation and expands the enzymatic toolkit for triterpenoid saponin biosynthesis.