噬菌体展示
抗体
单克隆抗体
免疫球蛋白轻链
重组DNA
肽库
抗原
抗体库
单链
分子生物学
生物
免疫学
化学
计算生物学
病毒学
生物化学
肽序列
基因
作者
Safar Farajnia,Vahideh Ahmadzadeh,Asghar Tanomand,Kamal Veisi,Shiva Ahdi Khosroshahi,Leila Rahbarnia
标识
DOI:10.3109/08923973.2014.945126
摘要
Recombinant antibodies are increasingly being employed as therapeutic agents especially in combination with anti-cancer drugs. The single-chain antibody fragments are small antigen-binding proteins which provide the most commonly used antibody formats for diagnostic and therapeutic purposes. These antibody fragments have more rapid tumor penetration and clearance from the serum relative to full-length monoclonal antibodies. There are in vitro antibody-display technologies such as phage display, cell surface display, ribosome display and mRNA display that can be used to isolate high specificity and affinity single-chain antibodies against a wide variety of targets. We review these strategies for generation of stable and active antibody fragments in the present article.
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