Antigen–Antibody Interaction‐Derived Bioadhesion of Bacterial Cellulose Nanofibers to Promote Topical Wound Healing

Abstract A practically helpful bioadhesion system can be developed for biomedical treatments by taking advantage of the antigen–antibody interaction in which an antibody binds to a protein with specific lock and key binding affinity. This study presents a new type of skin tissue adhesive system in which an involucrin antibody (SY5)‐conjugated bacterial cellulose nanofiber (BCNF) is bound to involucrin (IVL) in corneocytes of the stratum corneum. For this, the SY5 is covalently incorporated on the surface of carboxylate of 2,2,6,6‐tetramethylpiperidine‐1‐oxyl radical (TEMPO)‐oxidized BCNFs via the 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide (EDC)/ N ‐hydroxysuccinimide (NHS) coupling reaction. It is shown that the SY5‐conjugated BCNF (BCNF SY5 ) exhibits the antigen–antibody interaction with the IVL, eventually leading to effective adhesion to the skin surface. Moreover, it is demonstrated that the BCNF‐based skin adhesion promotes wound healing by basically providing a tissue environment where cell proliferation can occur actively. These results emphasize that the BCNF SY5 system could pave the way for developing a new type of tissue adhesive for skin tissue regeneration.