沸石咪唑盐骨架
咪唑酯
碳纳米管
化学
检出限
纳米颗粒
荧光
赭曲霉毒素A
材料科学
纳米技术
化学工程
色谱法
无机化学
有机化学
金属有机骨架
吸附
工程类
物理
真菌毒素
量子力学
食品科学
作者
Mengting Chen,Zexuan Liu,Yinyin Guan,Yuxuan Chen,Weipeng Liu,Yingju Liu
标识
DOI:10.1016/j.snb.2022.131609
摘要
The rational design of high-performance nanozyme is of great significance in biosensors. Here, using zeolitic imidazolate framework-8 (ZIF-8) as the precursor, the Co nanoparticle/N-doped carbon nanotubes (Co/NCNT) with hollow core-shell structure were prepared by high temperature pyrolysis of [email protected] imidazolate framework-67 ([email protected]), where [email protected] was not only functionalized as the source of N and C for growth of NCNTs but also provided the template to form the hollow structures. Encapsulation of Co nanoparticles (NPs) in NCNT can effectively anchor active sites and avoid self-agglomeration. The synergistic effect of Co NPs and NCNT is beneficial to increasing the oxidase-mimicking activity significantly, while the hollow structure promotes the binding of the active sites with the substrate to increase the reaction rates. Hence, the Co/NCNT possessed oxidase-mimicking activity to catalyze colorless 3,3′,5,5′-tetramethylbenzidine (TMB) without H2O2, while the blue TMB+ can also quench the fluorescence of AuAg nanoclusters (NCs) by the internal filter effect. A colorimetric-fluorescence dual-mode immunosensor was established to achieve sensitive detection of ochratoxin A (OTA) in a linear detection range from 0.001 to 10 μg/L with a low detection limit of 0.21 ng/L (colorimetric) and 0.17 ng/L (fluorescence), respectively. Due to the independence and mutual corroboration of the signals, the dual-mode immunoassay is more accurate and reliable than the single-mode immunoassay for OTA. Significantly, the strategy lays a solid foundation for the design of MOF-based nanozymes and innovative inspiration in food visual analysis.
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