孵化
γ-氨基丁酸
响应面法
味精
谷氨酸受体
产量(工程)
化学
氨基丁酸
生物化学
氨基酸
磷酸吡哆醛
神经递质
潜伏期
食品科学
色谱法
生物
酶
材料科学
受体
辅因子
冶金
作者
Hongbo Cai,Xuan Li,Daojie Li,Weiwei Liu,Yunsheng Han,Xiping Xu,Peilong Yang,Kun Meng
出处
期刊:Foods
[MDPI AG]
日期:2023-08-12
卷期号:12 (16): 3034-3034
被引量:1
标识
DOI:10.3390/foods12163034
摘要
Gamma-aminobutyric acid (GABA) is a widely available non-protein amino acid whose physiological importance goes beyond its role as an inhibitory neurotransmitter in mammals. The GABA synthesis ability of ten strains of Lactiplantibacillus plantarum was screened. They produced GABA ranging from 48.19 ± 3.44 to 100.75 ± 1.63 mg/L at 24 h-cultivation. Among them, Lp. plantarum FRT7 showed the highest GABA production. Therefore, FRT7 was chosen for GABA yield optimization. A one-factor-at-a-time strategy analysis of the GABA yield of FRT7 was performed, including the culture temperature, incubation time, inoculum volume, initial pH, the initial amount of monosodium glutamate (MSG), and pyridoxal 5'-phosphate (PLP) concentration, based on which the response surface methodology (RSM) was performed. After being cultured in an MRS culture medium supplemented with 3% MSG and 2 mmol/L of PLP at 40 °C with an initial pH of 7.0 for 48 h, the GABA reached a maximum yield of 1158.6 ± 21.22 mg/L. The results showed the experimental value of the GABA yield was in good agreement with the predicted values. Furthermore, the results from the RSM also indicated that the initial MSG addition, PLP concentration, and incubation time were significant variables. These results suggest that Lp. plantarum FRT7 has the potential to be a health-beneficial probiotic with commercial capabilities.
科研通智能强力驱动
Strongly Powered by AbleSci AI