Nucleic acid-aided molecular amplification techniques for food microorganism detection

The safety in agriculture, food and medicine production and processing is of grave importance to global economic and social stability. Efficient techniques for microorganism detection are essential to control health risks during product circulation. Rapid development in functional nucleic acids (FNAs) and molecular amplification technologies with improvements in the operation time, sensitivity and high throughput analysis have brought about a qualitative leap in microorganism detection. This review gives an investigative summary of many novel microorganism detection methods driven by nucleic acid-aided molecular amplification techniques (NAAMAT) in three aspects: direct to indirect methods, simple to integrated strategies, and representative applications. This paper will provide a useful reference and guide for future development of more advanced nucleic acid-aided molecular amplification techniques.