Imidazo-pyridine-based zinc(ii) complexes as fluorescent hydrogen sulfide probes

In this work we explore the interaction of HS^- with a family of fluorescent zinc complexes. In particular we selected a family of complexes with N,O-bidentate ligands aiming at assessing whether the zinc-chelating ligand plays a role in influencing the reactivity of HS^- with the complexes under investigation. Different experiments, performed by diverse spectroscopic techniques, provide evidence that HS^- binds the zinc center of all the complexes included in this study. The results highlight the potential of the devised systems to be used as HS^-/H₂S fluorescent sensors via a coordinative-based approach. To shed light on the species formed in solution when HS^-/H₂S interacts with the title complexes and aiming to rationalize the photophysical properties of the sensing constructs, we performed a computational analysis based on the time dependent density functional theory (TD-DFT). Preliminary bio-imaging experiments were also performed and the results indicate the potential of this class of compounds as probes for the detection of H₂S in living cells.