谷胱甘肽
细胞内
氧化物
活力测定
石墨烯
细胞生物学
生物物理学
细胞
材料科学
化学工程
生物化学
纳米技术
生物
冶金
工程类
酶
作者
Baojin Ma,Shi Guo,Yuta Nishina,Alberto Bianco
标识
DOI:10.1021/acsami.0c17523
摘要
Graphene oxide (GO) is currently developed for biomedical applications as a promising nanoplatform for drug delivery, phototherapy, and biosensing. As a consequence, its safety and cytotoxicity issues have attracted extensive attention. It has been demonstrated that GO causes an increase of intracellular oxidative stress, likely leading to its cytotoxicity and inhibition of cell proliferation. Being one of the main reductive intracellular substances, glutathione (GSH) is vital in the regulation of the oxidative stress level to maintain normal cellular functions. In this study, we found that GSH could be oxidized to GSSG by GO, leading to the formation of reduced GO (rGO). GSH depletion affects the intracellular reductive/oxidative balance, provoking the increase of the reactive oxygen species level, sequentially inhibiting cell viability and proliferation. Therefore, the reaction between GO and GSH provides a new perspective to explain the origin of GO cytotoxicity.
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