Lipid metabolism changes in C. elegans models of aging

Lipid metabolism plays major roles in cellular membrane structure and fluidity, signaling, and energy storage. Lipids have a tremendously diverse composition, structures, and saturation levels, making their cellular function complex. Recent data have suggested that presence of particular lipids correlate with aging physiology, including cardiovascular and neurodegenerative diseases. Furthermore, genetic or pharmacological manipulation of lipid enzyme function impacts aging, including lifespan and healthspan (period of life in good health). Using genetic models of aging in Caenorhabditis elegans , we performed a lipidomics study examining a panel of lipids, including free fatty acids, triacylglycerols, sphingomyelins, and other phospholipids. We measured 888 lipids, and 702 lipids were present in 80% of our samples. We tracked these lipids in 1, 5, and 10 day old adult worms, and analyzed patterns across age and mutant background. In particular, we highlight findings in a sphingolipid metabolism mutant, asm‐3 (acid sphingomyelinase), which has an increased lifespan. We compare these to physiological responses of asm‐3 mutants in response to stress. Together, we hope this analysis adds to our understanding of the biology of aging and yields novel lipid targets that may impact lifespan and healthspan. Support or Funding Information NIH 2R15AG052933 and NIH 1S10OD021562