Size Fractionation of plant Protoplasts by Centrifugal Elutriation

Centrifugal elutriation is a technique (1) used to separate particles primarily on the basis of size by balancing centrifugal force against flow of medium in the opposite direction (Fig. 1). Particles of similar density but different size will band in an elutriation chamber at different positions, and different fractions can be eluted from the chamber first followed by progressively larger particles.