色谱法
不应报告的
化学
皂化
气相色谱法
毛细管作用
重氮甲烷
毛细管气相色谱法
溶剂
有机化学
材料科学
复合材料
作者
Hal T. Slover,Raymond H. Thompson,George V. Merola
摘要
Abstract A method is described for simultaneously determining tocopherols and sterols in fats and oils by quantitative capillary gas chromatography. Samples containing ca. 100 mg of lipid were saponified in capped tubes with aqueous KOH by heating for 8 min at 80 C; the unsaponifiable fraction was extracted with cyclohexane, freed of solvent, derivatized to form the trimethylsilyl ethers of both tocopherols and sterols, and chromatographed on a 50 m×0.25 mm glass capillary column coated with Dexsil 400. Most of the individual tocopherols and common sterols were well separated, although interfering peaks were seen in some samples, which for better specificity should be subjected to an initial purification. For most samples, however, the simplified sample preparation, without preliminary purification, was adequate when combined with capillary gas chromatography. Recovery, method and gas liquid chromatographic precision, and applications are discussed.
科研通智能强力驱动
Strongly Powered by AbleSci AI