Simultaneous HPTLC analysis of flavonoids in the leaves of three different species of bamboo

Flavonoids are the main functional compounds in a bamboo leaf extract. Because of their activity flavonoids are widely used in many of health products, cosmetics, and food supplements. A simple high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous quantification of vitexin, isovitexin, orientin, and isoorientin in the leaves of three different bamboo species: Phyllostachys pubescens Mazel ex H. de Lehaie, Phyllostachys glauca McClure, and Pleioblastus yixingensis S. L. Chen et S. Y. Chen. The method was validated for precision (expressed as the coefficient of variation, CV [%]), accuracy, sensitivity, and selectivity. Instrumental precision was found to be 0.37, 0.52, 0.39, 0.13, and 0.84% and the repeatability of the method 0.98, 0.91, 1.02, 1.04 and 0.87% for isovitexin, rutin, orientin, isoorientin, and vitexin, respectively. The accuracy of the method was determined by measurement of recovery at three different concentrations. Average recovery was 99.54% for ...