期刊:CSH Protocols [Cold Spring Harbor Laboratory] 日期:2007-09-01卷期号:2007 (9)被引量:10
标识
DOI:10.1101/pdb.prot4826
摘要
INTRODUCTIONEarly Drosophila embryos are particularly amenable to cellular analysis. However, they are protected by an outer chorion layer, as well as an impermeable and opaque vitelline membrane. Consequently, preparation of Drosophila embryos for fixed-sample analysis must include procedures for chorion removal, vitelline membrane permeabilization, fixation, and finally, vitelline membrane removal. This protocol describes a method for removing the chorion layer.