登革热
计算生物学
双杂交筛选
鉴定(生物学)
生物
发病机制
酵母
病毒学
免疫学
遗传学
植物
作者
Jonas Nascimento Conde
出处
期刊:Springer eBooks
[Springer Nature]
日期:2021-10-29
卷期号:: 119-132
标识
DOI:10.1007/978-1-0716-1879-0_9
摘要
Yeast two-hybrid (Y2H) systems are one of the principal choices for identifying novel binary protein-protein interactions (PPIs). Since its development, it has contributed for the discovery of several PPIs between pathogens and host, allowing not only a comprehensive look at the disease pathogenesis but also for therapeutic strategies. Identification of viral-host PPIs that impact on viral replication and pathogenesis can lead to new advances in antiviral therapies such as the development of drug candidates and vaccine design. In this chapter, we revise the Y2H key parameters necessary for screening PPIs and discuss the possible approaches for using this technique to identify novel dengue-host protein interactions.
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