化学
霉酸
质谱法
结核分枝杆菌
离子阱
加合物
碎片(计算)
立体化学
色谱法
有机化学
肺结核
计算机科学
医学
操作系统
病理
作者
Cheryl Frankfater,Hideji Fujiwara,Spencer J. Williams,Adriaan J. Minnaard,Fong‐Fu Hsu
标识
DOI:10.1021/jasms.1c00310
摘要
Mycobacterium tuberculosis (Mtb) cells are known to synthesize very long chain (C60–90) structurally complex mycolic acids with various functional groups. In this study, we applied linear ion-trap (LIT) multiple-stage mass spectrometry (MSn), combined with high-resolution mass spectrometry to study the mechanisms underlying the fragmentation processes of mycolic acid standards desorbed as lithiated adduct ions by ESI. This is followed by structural characterization of a Mtb mycolic acid family (Bovine strain). Using the insight fragmentation processes gained from the study, we are able to achieve a near complete characterization of the whole mycolic acid family, revealing the identity of the α-alkyl chain, the location of the functional groups including methyl, methoxy, and keto groups along the meroaldehyde chain in each lipid species. This study showcased the power of LIT MSn toward structural determination of complex lipids in a mixture, which would be otherwise very difficult to define using other analytical techniques.
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