Dual‐Cross‐Linked Chitosan‐Based Antibacterial Hydrogels with Tough and Adhesive Properties for Wound Dressing

Abstract Biocompatible chitosan‐based hydrogels have attracted extensive attention in wound dressing due to their human skin‐like tissue characteristics. However, it is a crucial challenge to fabricate chitosan‐based hydrogels with versatile properties, including flexibility, stretchability, adhesivity, and antibacterial activity. In this work, a kind of chitosan‐based hydrogels with integrated functionalities are facilely prepared by solution polymerization of acrylamide (AAm) and sodium p ‐styrene sulfonate (SS) in the presence of quaternized carboxymethyl chitosan (QCMCS). Due to the dual cross‐linking between QCMCS and P(AAm ‐co‐ SS), the optimized QCMCS/P(AAm‐ co ‐SS) hydrogel exhibits tough mechanical properties (0.767 MPa tensile stress and 1100% fracture strain) and moderate tissue adhesion (11.4 kPa). Moreover, biological evaluation in vitro illustrated that as‐prepared hydrogel possesses satisfactory biocompatibility, hemocompatibility, and excellent antibacterial ability (against S. aureus and E. coli are 98.8% and 97.3%, respectively). Then, the hydrogels are tested in a rat model for bacterial infection incision in vivo, and the results show that they can significantly accelerate epidermal regeneration and wound closure. This is due to their ability to reduce the inflammatory response, promote the formation of collagen deposition and granulation tissue. The proposed chitosan‐based antibacterial hydrogels have the potential to be a highly effective wound dressing in clinical wound healing.