Overview of Analytical Methods for Characterizing the Charge Heterogeneity of Antibody-Drug Conjugates

Antibody-drug conjugates (ADCs) are ideal candidates for selective therapy. ADCs display considerable heterogeneity resulting from various modifications. One of the molecular characteristics of ADCs is charge heterogeneity. Several analytical methods could be used to characterize the charge heterogeneity of ADC including ion exchange chromatography (IEC), isoelectric focusing (IEF), and capillary electrophoresis (CE). This work aimed to give insights into the different analytical techniques used to determine pI and the charge distribution profile of ADCs.