Effects of Combination of G-CSF and SCF One Week Prior to Liver Injury In Acute liver Damage Model Induced by Thioacetamide Administration

Background: There are many reports regarding to effects of Granulocyte colonystimulating factor (G-CSF) and stem cell factor (SCF)alone in liver repair .But conflicting data have been reported regarding the role of growth factors such as G-CSF and SCF in the liver regeneration system.Also, there is not such data regarding to effects of co-administration both of G-CSF and SCF in the liver damage condition.Materials and Methods: An experimental model of rat liver damage induced by the thioacetamide.Five different groups of animals receiving 0.9% NaCl, TAA alone, TAA + G-CSF, TAA + SCF and TAA + (G-CSF+SCF ).The activity of glutamate pyruvate transaminase (GPT/AlT)and glutamate oxaloacetate transaminase (GOT/AST) were measured after the thioacetamide (TAA) injection and the administration of combination of G-CSF +SCF for 12 weeks.Also histological tests were carried out at the end experiments.Results: The pre-treatment of combination of G-CSF and SCF for 12 weeks reduced the degree of liver injury.The mean of GOT activity was 61.24 (U/L) in the G-CSF +SCF and versus 132.86 in the TAA-alone group.These differences in the GOT activity were statistically significant (P<0.05).Also, in the G-CSF +SCF and TAA group the mean of GPT activity (4.35 versus 11.79, respectively) were lower than in the TAA-alone group, this difference was statistically significant (P<0.05).Liver sections from a rat treated only with TAA, showing damage, but TAA and G-CSF + SCF no significant damage is present.On the other hand histological results revealed a very mild degree of inflammation were observed in the livers of the combination of G-SCF+SCF and TAA-treated rats compared to TAA only treated group.Conclusion: Biochemical and microscopic analysis revealed that combination of G-CSF and SCF pre-treatment significantly enhances liver regeneration after TAA-induced liver injury.