Hydrophobic Interaction Chromatography

Abstract Hydrophobic interaction chromatography involves the separation of protein molecules in their native and biologically active state owing to the differential interaction of these molecules with hydrophobic sites on the surface of a solid support. In the separation process, hydrophobic patches on the protein interact with hydrophobic molecules (e.g. alkyl residues) immobilized on the hydrophilic solid‐phase surface (agarose).