生物
发起人
RNA聚合酶Ⅱ
RNA聚合酶
聚合酶
遗传学
酵母
分子生物学
转录因子ⅡD
RNA聚合酶Ⅰ
RNA依赖性RNA聚合酶
计算生物学
基因表达
核糖核酸
基因
作者
Paul D. Good,David R. Engelke
出处
期刊:Gene
[Elsevier]
日期:1994-12-01
卷期号:151 (1-2): 209-214
被引量:32
标识
DOI:10.1016/0378-1119(94)90658-0
摘要
A series of Saccharomyces cerevisiae--Escherichia coli shuttle vectors is described in which small RNAs can be stably expressed in yeast from two different promoters for RNA polymerase III transcription. The vectors are available in either high- or low-copy-number forms with either URA3, HIS3, or TRP1 selection markers, and are based on a previously described set of plasmid vectors [Sikorski and Hieter, Genetics 122 (1989) 19-27]. Transcripts have structured pre-tRNA or RPR1 leaders fused to RNA corresponding to inserted sequences. Levels of RNA accumulation are dependent on plasmid copy number and the type of transcript.
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