Recent progress in the development of derivatization reagents having a benzofurazan structure

Chemical derivatization is often used to enhance the detectability of the target compounds and to improve the separation efficiency in high-performance liquid chromatography (HPLC). In this review, we describe the recent progress in the development of derivatization reagents having a benzofurazan structure, namely, the fluorogenic reagents, water-soluble reagents, reagents for the analysis of peptides and proteins, and reagents for mass spectrometric detection. The application of these reagents to bio-samples is also briefly described.