Study on preparation of folate-conjugated fluorescein-loaded album nanoparticles

Purpose To study on the preparation of folate-conjugated fluorescein-loaded album nanopaticles.Methods The bovine serum albumin nanoparticles(BSANPs) were prepared by desolvation method and chemical cross-linking with glutaraldehyde.The activated folic acid(N-hydroxysuccinimide ester of folic acid) was conjugated to the surface of BSANPs via the amino groups.Then fluorescein was incorporated of into the folate-conjugated BSANPs,purifying with Sephadex G-50 column.The extent of fluorescein loaded on the folate-BSANPs was determined by UV spectrophotometic analysis.Results The fluorescein was successfully loaded into the folate-conjugated album nanopaticles,while the entrapment rate of fluorescein is 6.4%.Conclusion Folate-conjugated fluorescein-loaded album nanopaticles were achieved,which represent a new future of loading the near-infrared fluorescein into folate-conjugated album nanopaticles.