In this paper,the decoloring of didromocarboxysenazo oxidized by cerium(Ⅳ) in sulfuric acid has been studied.The decrease in absorbance is directly proportional to the amount of cerium.Under the reaction temperature of 25℃,The maxium absorption is at 530nm,the detection limit was 1.66×10~(-6) μg/mL and the regressive eqution is :ΔA=0.1646C_(Ce(Ⅳ))(μg/mL)-0.0194 and the liner range of the determination was 0~8.5 μg/mL.The method has been used for the determination of cerium in hair with satisfactory results.