染色质免疫沉淀
免疫沉淀
芯片对芯片
染色质
细胞生物学
组蛋白
芯片排序
转录因子
核蛋白
DNA结合蛋白
嘉雅宠物
生物
染色质重塑
化学
DNA
分子生物学
遗传学
基因表达
基因
发起人
作者
Berthe Katrine Fiil,Jin‐Long Qiu,Kathrine Birch Petersen,Morten Petersen,John Mundy
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2008-09-01
卷期号:2008 (9): pdb.prot5049-pdb.prot5049
被引量:34
摘要
INTRODUCTIONTranscriptional reprogramming occurs during development and in response to diverse stimuli and stresses. The isolation and characterization of nuclear proteins, particularly those binding to DNA and chromatin, are therefore important to understanding these processes. Two specific approaches to understanding the function of nuclear proteins involve the characterization of their protein-protein interactions, and of the transcriptional targets of specific transcription factors. Coimmunoprecipitation (co-IP) is a straightforward technique to study in vivo protein-protein interactions, and can identify interacting proteins or protein complexes present in cell extracts. Chromatin immunoprecipitation (ChIP) permits the identification of protein-DNA interactions in pull-down assays using specific antibodies against DNA-binding proteins, such as transcription factors or histone/chromatin-binding proteins. Here, we present detailed protocols for extraction of Arabidopsis seedlings, co-IP of nuclear proteins, and ChIP.
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