生物
白细胞介素-7受体
祖细胞
造血
单核细胞
免疫学
细胞生物学
胎儿
祖细胞
干细胞
T细胞
遗传学
免疫系统
白细胞介素2受体
怀孕
作者
Gabriel Leung,Taylor Cool,Clint H. Valencia,Atesh Worthington,Anna E. Beaudin,Camilla Forsberg
出处
期刊:Development
[The Company of Biologists]
日期:2019-07-15
卷期号:146 (14)
被引量:61
摘要
ABSTRACT The discovery of a fetal origin for tissue-resident macrophages (trMacs) has inspired an intense search for the mechanisms underlying their development. Here, we performed in vivo lineage tracing of cells with an expression history of IL7Rα, a marker exclusively associated with the lymphoid lineage in adult hematopoiesis. Surprisingly, we found that Il7r-Cre labeled fetal-derived, adult trMacs. Labeling was almost complete in some tissues and partial in others. The putative progenitors of trMacs, yolk sac (YS) erythromyeloid progenitors, did not express IL7R, and YS hematopoiesis was unperturbed in IL7R-deficient mice. In contrast, tracking of IL7Rα message levels, surface expression, and Il7r-Cre-mediated labeling across fetal development revealed dynamic regulation of Il7r mRNA expression and rapid upregulation of IL7Rα surface protein upon transition from monocyte to macrophage within fetal tissues. Fetal monocyte differentiation in vitro produced IL7R+ macrophages, supporting a direct progenitor-progeny relationship. Additionally, blockade of IL7R function during late gestation specifically impaired the establishment of fetal-derived trMacs in vivo. These data provide evidence for a distinct function of IL7Rα in fetal myelopoiesis and identify IL7R as a novel regulator of trMac development.
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