DEVELOPMENT AND APPLICATION OF NEW RP-HPLC-DAD METHOD FOR THE QUANTIFICATION OF SOME BETA BLOCKERS IN COMMERCIAL FORMULATIONS, SPIKED WATER AND BIOLOGICAL SAMPLES

The excessive use and over dose of beta blockers used for the treatment of various cardiovascular disorders can lead to several health problems. This necessitates new methodologies for the determination of these drug compounds. A simple, selective and precise RP-HPLC method was developed and validated for the assay of some beta blockers in commercial formulations, spiked water and biological samples. Gradient reverse phase elution of mobile phase comprising of methanol and water (90:10) with optimized flow rate of 0.8 mL min-1 on a low carbon loaded TSK 250-Biogel C-8, Phenyl-X Gel-7PW, HPLC column (150mm X 3.5?m, i.d. 4.6mm) was controlled to 65 minutes after complete resolution of the peaks present in the matrix without any interferences with the neighboring excipients with least HETP value. Beta blockers viz. atenolol, esmolol, propranolol and metoprolol were analyzed and quantified at retention time 30.1, 29.8, 33.4 and 34.2 minutes respectively at their corresponding absorption…