Gender-Related Differences in Tissue Distribution, Excretion, and Metabolism Studies of Panaxadiol in Rats and Anti-inflammatory Study

In this study, we evaluated gender differences in PD excretion, tissue distribution, and metabolism in rats. In addition, we also evaluated its anti-inflammatory activity and mechanism. The results showed that the concentrations of PD in the stomach, small intestine, and large intestine were the highest. The C_max of female rats was significantly higher than that of male rats. With regard to genital tissues, the C_max of PD in the uterus and ovary was higher than that in the testis. In the excretion test, gender had no significant effect on the excretion of PD. Its total excretion in rats was about 30%. Therefore, we speculated 12 phase I metabolites. In the anti-inflammatory test, PD showed no cytotoxic effect on macrophage RAW 264.7 and significantly reduced the production of NO and expressions of interleukin 6, interleukin 1, and tumor necrosis factor-α. Further analyses demonstrated that PD activated the MAPK signaling pathway by reducing the phosphorylated levels of p38 and ERK.