叶绿素荧光
光系统II
荧光
光合作用
莱茵衣藻
猝灭(荧光)
紫黄质
荧光寿命成像显微镜
生物物理学
非光化学猝灭
光化学
化学
生物
植物
玉米黄质
突变体
光学
类胡萝卜素
物理
生物化学
基因
叶黄素
作者
Oliver Holub,Manfredo J. Seufferheld,Christopher Gohlke,. Govindjee,Robert M. Clegg
出处
期刊:Photosynthetica
[Institute of Experimental Botany]
日期:2000-11-01
卷期号:38 (4): 581-599
被引量:87
标识
DOI:10.1023/a:1012465508465
摘要
We describe an instrument that allows the rapid measurement of fluorescence lifetime-resolved images of leaves as well as sub-cellular structures of intact plants or single cells of algae. Lifetime and intensity fluorescence images can be acquired and displayed in real time (up to 55 lifetime-resolved images per s). Our imaging technique therefore allows rapid measurements that are necessary to determine the fluorescence lifetimes at the maximum (P level) fluorescence following initial illumination during the chlorophyll (Chl) a fluorescence transient (induction) in photosynthetic organisms. We demonstrate the application of this new instrument and methodology to measurements of: (1) Arabidopsis thaliana leaves showing the effect of dehydration on the fluorescence lifetime images; (2) Zea mays leaves showing differences in the fluorescence lifetimes due to differences in the bundle sheath cells (having a higher amount of low yield photosystem 1) and the mesophyll cells (having a higher amount of high yield photosystem 2); and (3) single cells of wild type Chlamydomonas reinhardtii and its non-photochemical quenching mutant NPQ2 (where the conversion of zeaxanthin to violaxanthin is blocked), with NPQ2 showing lowered lifetime of Chl a fluorescence. In addition to the lifetime differences referred to in (1) and (2), structural dependent heterogeneities in the fluorescence lifetimes were generally observed when imaging mesophyll cells in leaves.
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