拟南芥
砷酸盐
拟南芥
细胞生物学
钙
生物
磷酸化
运输机
生物化学
信号转导
突变体
化学
砷
基因
有机化学
作者
Yisong Liu,Yanting Zhang,Zhangqing Wang,Shuwen Guo,Yanjun Fang,Zhenqian Zhang,Huiling Gao,Huimin Ren,Cun Wang
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2023-03-21
卷期号:192 (2): 910-926
被引量:6
标识
DOI:10.1093/plphys/kiad171
摘要
Arsenate [As(V)] is a metalloid with heavy metal properties and is widespread in many environments. Dietary intake of food derived from arsenate-contaminated plants constitutes a major fraction of the potentially health-threatening human exposure to arsenic. However, the mechanisms underlying how plants respond to arsenate stress and regulate the function of relevant transporters are poorly understood. Here, we observed that As(V) stress induces a significant Ca2+ signal in Arabidopsis (Arabidopsis thaliana) roots. We then identified a calcium-dependent protein kinase, CALCIUM-DEPENDENT PROTEIN KINASE 23 (CPK23), that interacts with the plasma membrane As(V)/Pi transporter PHOSPHATE TRANSPORTER 1;1 (PHT1;1) in vitro and in vivo. cpk23 mutants displayed a sensitive phenotype under As(V) stress, while transgenic Arabidopsis plants with constitutively active CPK23 showed a tolerant phenotype. Furthermore, CPK23 phosphorylated the C-terminal domain of PHT1;1, primarily at Ser514 and Ser520. Multiple experiments on PHT1;1 variants demonstrated that PHT1;1S514 phosphorylation is essential for PHT1;1 function and localization under As(V) stress. In summary, we revealed that plasma-membrane-associated calcium signaling regulates As(V) tolerance. These results provide insight for crop bioengineering to specifically address arsenate pollution in soils.
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