Development of Anti-aging Cosmetics Using Chitosan/ γ-Cyclodextrin/Fucoidan Nanoparticles (Nanogel) Based on the Drug Delivery System

Purpose: This study aimed to develop MARINAVI-FD, a cosmetic material containing γ-cyclodextrin/chitosan/fucoidan nanoparticles, i.e., CH-γ-CD-Fu (Nanogel) based on the DDS system, and anti-aging cosmetics that have proven antioxidant and moisturizing effects.Methods: CH-γ-CD-Fu (nanogel) was identified through electron microscope (scanning electron microscope) analysis and nanoparticle size analysis (dynamic light scattering). Radical scavenging DPPH and ABTS assays were performed. Skin keratinocytes (HaCat cells) was assessed by the MTT assay, and the moisturizing effect was evaluated through enzyme-linked immunosorbent assay. Temperature and storage conditions were checked for the stability of the formulation. In the human body application test, Moisture Meter D Compact device was used.Results: The CH-γ-CD-Fu (Nanogel) has confirmed particle size of approximately 500 nm. In the DPPH and ABTS assays, compared with Fu (foucoidan), the CH-γ-CD-Fu (nanogel) showed higher scavenging ability at treatment concentration of 15.625–500 μg/mL. CH-γ-CD-Fu (Nanogel) showed a survival rate of >80% in skin keratinocytes (HaCat cells) up to a concentration of 5,000 μg/mL. In the measurement of hyaluronic acid production, CH-γ-CD-Fu (Nanogel) showed better production at 194.87 ng/mL than at 121.47 ng/mL at a concentration of 200 μg/mL in the control group. The stability of skin and cream formulations of anti-aging cosmetics containing MARINAVI-FD was confirmed. The moisturizing effect was observed 2.5 mm depth of skin human application.Conclusion: In this study, CH-γ-CD-Fu (nanogel) was used to materialize into anti-aging cosmetics.