加压器
拟南芥
抑制因子
生物
开花位点C
突变体
基因
遗传学
拟南芥
菊花
转录调控
基因表达
细胞生物学
植物
作者
Zixin Zhang,Qian Hu,Zhengyuan Gao,Yuqing Zhu,Mengru Yin,Erlei Shang,Gaofeng Liu,Weixin Liu,Rongqian Hu,Hongfei Cheng,Xinran Chong,Zhouguo Guan,Weimin Fang,Sumei Chen,Bo Sun,Yuehui He,Fadi Chen,Jiafu Jiang
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2023-08-30
卷期号:193 (4): 2413-2429
被引量:3
标识
DOI:10.1093/plphys/kiad476
摘要
Abstract Plant flowering time is induced by environmental and endogenous signals perceived by the plant. The MCM1-AGAMOUSDEFICIENS-Serum Response Factor-box (MADS-box) protein SHORT VEGETATIVE PHASE (SVP) is a pivotal repressor that negatively regulates the floral transition during the vegetative phase; however, the transcriptional regulatory mechanism remains poorly understood. Here, we report that CmSVP, a chrysanthemum (Chrysanthemum morifolium Ramat.) homolog of SVP, can repress the expression of a key flowering gene, a chrysanthemum FLOWERING LOCUS T-like gene (CmFTL3), by binding its promoter CArG element to delay flowering in the ambient temperature pathway in chrysanthemum. Protein–protein interaction assays identified an interaction between CmSVP and CmTPL1-2, a chrysanthemum homologue of TOPLESS (TPL) that plays critical roles as transcriptional corepressor in many aspects of plant life. Genetic analyses revealed the CmSVP-CmTPL1-2 transcriptional complex is a prerequisite for CmSVP to act as a floral repressor. Furthermore, overexpression of CmSVP rescued the phenotype of the svp-31 mutant in Arabidopsis (Arabidopsis thaliana), overexpression of AtSVP or CmSVP in the Arabidopsis dominant-negative mutation tpl-1 led to ineffective late flowering, and AtSVP interacted with AtTPL, confirming the conserved function of SVP in chrysanthemum and Arabidopsis. We have validated a conserved machinery wherein SVP partially relies on TPL to inhibit flowering via a thermosensory pathway.
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