细胞色素c
采后
烟草
程序性细胞死亡
生物
细胞色素
叶绿素
甘露糖
衰老
生物化学
活力测定
叶绿体
细胞凋亡
细胞生物学
植物
分子生物学
基因
酶
作者
Shiying Zhao,Jiahui Chen,Shifeng Cao,Hongfei Wang,Hangjun Chen,Yingying Wei,Xingfeng Shao,Xingfeng Shao,Feng Xu
标识
DOI:10.1016/j.plaphy.2024.108480
摘要
It is well established that programmed cell death (PCD) occurred in broccoli during postharvest senescence, but no studies have been conducted on the regulation of broccoli cytochrome f by mannose treatment and its relationship with PCD. In this study, we treated broccoli buds with mannose to investigate the changes in color, total chlorophyll content, gene expression related to chlorophyll metabolism, chloroplast structure, and cytochrome f determination during postharvest storage. In addition, to investigate the effect of cytochrome f on PCD, we extracted cytochrome f from broccoli and treated Nicotiana tabacum L. cv Bright Yellow 2 (BY-2) cells with extracted cytochrome f from broccoli at various concentrations. The results showed that cytochrome f can induce PCD in tobacco BY-2 cells, as evidenced by altered cell morphology, nuclear chromatin disintegration, DNA degradation, decreased cell viability, and increased caspase-3-like protease production. Taken together, our study indicated that mannose could effectively delay senescence of postharvest broccoli by inhibiting the expression of gene encoding cytochrome f which could induce PCD.
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