脱氧核酶
基因沉默
化学
生物物理学
G-四倍体
小干扰RNA
纳米技术
细胞生物学
RNA干扰
组合化学
核糖核酸
核酸
基因传递
DNA
生物化学
基因
材料科学
生物
作者
Huanhuan Fan,Zilong Zhao,Guobei Yan,Xiaobing Zhang,Chao Yang,Hong-Min Meng,Zhuo Chen,Hui Liu,Weihong Tan
标识
DOI:10.1002/anie.201411417
摘要
DNAzymes hold promise for gene-silencing therapy, but the lack of sufficient cofactors in the cell cytoplasm, poor membrane permeability, and poor biostability have limited the use of DNAzymes in therapeutics. We report a DNAzyme–MnO2 nanosystem for gene-silencing therapy. MnO2 nanosheets adsorb chlorin e6-labelled DNAzymes (Ce6), protect them from enzymatic digestion, and efficiently deliver them into cells. The nanosystem can also inhibit 1O2 generation by Ce6 in the circulatory system. In the presence of intracellular glutathione (GSH), MnO2 is reduced to Mn2+ ions, which serve as cofactors of 10–23 DNAzyme for gene silencing. The release of Ce6 generates 1O2 for more efficient photodynamic therapy. The Mn2+ ions also enhance magnetic resonance contrast, providing GSH-activated magnetic resonance imaging (MRI) of tumor cells. The integration of fluorescence recovery and MRI activation provides fluorescence/MRI bimodality for monitoring the delivery of DNAzymes.
科研通智能强力驱动
Strongly Powered by AbleSci AI