Isolation and purification of thymosin β_4 from calf thymus

Objective:To isolate and purify thymosin β4 from calf thymus.Methods: Thymosin fraction 5 was extracted by ammonium sulfate precipitation.Thymosin β4 was purified by ion exchange chromatography,and elucidated by SDS-PAGE and HPLC.Results:The purity of thymosin β4 was 90.1%.Conclusion:Thymosin β4 was purified by ion exchange chromatography and warranted for the pilot manufacture.