Properties comparing and evolutionary analysis of MEF2 of <I>Homo sapiens</I> based on bioinformatic methods

As one of the members of MADS family, MEF2 group is important in regulating development. Analytical tools of NCBI, ExPASy, CBS, CDD, and SABLE were adopted to analyze the properties of human MEF2 proteins, and evolutionary tree was built according to the result of correlative sequence alignments. The results showed that there are various forms of MEF2 in human body, and there are some differences in the physicochemical characteristics. Relatively more phosphorylation sites are found and the main glycosylation sites are N-glycosylation sites. All MEF2 proteins of human contain MADS domain, and most contain MEF2 domain and HJURP_C domain. Their secondary structures contain three dominant states: helix, sheet and coil, their tertiary structures are similar. The phylogenetic tree result shows that MEF2B may be original because of its difference of sequences and evolutional relation.