In this study,the stepwise behavioral responses of the Japanese medaka(Oryzias latipes)under the joint stress of two herbicides(Atrazine and Paraquat)were discussed.BEWs was used to record the data of behavior strength to analyze the behavioral responses of Japanese medaka.The evident difference of the behavioral responses time were:0.74、 7.7、29.4 and 42.2h,and evident difference of the behavioral responses time was detected.The results suggested that the stepwise behavioral responses of the Japanese medaka were affected by both the chemical concentration and exposure time.And the evident difference of the behavioral response time was detected.The stepwise behavioral responses in different proportion of the two herbicides were almost the same as each other.It proved that the toxicity effects of the joint stress of the two herbicides on the stepwise behavioral responses of Japanese medaka were synergistic.