PREPARATION AND IDENTIFICATION OF CAMEL HEAVY CHAIN ANTIBODY SPECIFIC POLYCLONAL ANTIBODY

Purpose: In addition to conventional antibody Ig G1 in serum of camel,there are two subtypes of heavy chain antibody Ig G2 and Ig G3,they have the advantages of small molecular weight and strong stability,but the research and application of camel heavy chain antibody was certainly restricted since lacking of efficient detection techniques. It has some application prospect to prepare the polyclonal antibody that specific for camel heavy chain antibody. Method: In this experiment,camel plasma was obtained by Mararating lymphocytes from the camel whole blood with lymphocytes isolation kit,and then the three subtype antibody of camel was Mararated and purified through Protein G / A Resin column. After three times immunization of rabbit with camel Ig G2,the rabbit polyclonal antibodies were prepared in allusion to heavy chain antibody of camels,and its activity was identified. Result: The result of SDS- PAGE gel electrophoresis showed that camel subtype antibodies were Mararated and purified successfully. Indirect ELISA results showed the titer of polyclonal antibody was 1: 4 096. By combining Indirect ELISA and Western- blot determine the specificity of the antibody,we got that the polyclonal antibody has no any cross reaction with cattle Ig G and camel Ig G1,but has reaction with Ig G3. Conclusion: The experimental results show that we prepared camel heavy chain antibody specific polyclonal antibody successfully,and it will provide strong support for the development and utilization of camel antibody resources,as well as camel disease detection and research.