Relationship on mRNA of lysyl oxidase and elastin with pelvic organ prolapse

Objective To investigate the expression of lysyl oxidase( LOX) and elastin in anterior vaginal wall of women with pelvic organ prolapse( POP) and the relationships with POP. Methods The anterior vaginal wall samples were obtained from 39 POP patients undergoing anterior colporrhaphy or total hysterectomy,including 12 premenopausal cases due to vaginocele or uterine prolapse and 27 postmenopausal cases. The other anterior vaginal wall samples were obtained from 40 non-POP women( control group) undergoing total hysterectomy due to benign gynecological diseases,non-stress urinary incontinence or POP. Real-time fluorescence quantitation polymerase chain reaction( PCR) was used to verify the mRNA level of LOX and elastin. Results The premenopausal and postmenopausal POP groups demonstrated significantly decreasing expressions of LOX,lysyl oxidase like 1( LOXL1) and elastin in anterior vaginal wall compared with control group( P 0. 05). In POP group,the mRNA expressions of LOX,LOXL1 and elastin in postmenopausal patients were significantly lower than those in premenopausal patients( P 0. 05). There was a positive correlation between LOX and LOXL1 mRNA with elastin mRNA in POP group( r = 0. 936 1 and 0. 689 8,P 0. 05).Conclusions The decreasing expressions of LOX and LOXL1 in the anterior vaginal wall may contribute to POP.