Simple, Rapid and Sensitive Method for Determination of Tacrolimus in Human Blood by using Liquid Chromatography/Tandem Mass Spectrometry

A simple, rapid and sensitive method using an isocratic Liquid chromatography coupled with Tandem mass spectrometry was developed and validated for the assay of Tacrolimus in the Human Blood. The Mass transition of Tacrolimus and Sirolimus (Internal standard) were M/z 821.7/