FITC labeling of bacteria

Hey Cindy, Here is the protocol for preparation and imaging of bacteria for fluorescence microscopy (Growth Curve protocol--see Step 9 under Section II). I also attached a protocol for FITC labeling of bacteria, which you will need once you start the phagocytosis assays. I’m not emailing the phagocytosis protocol since we will probably need to develop a personalized protocol for your studies. K