Engineered ketoreductase-catalyzed stereoselective reduction of ethyl 2′-ketopantothenate and its analogues: chemoenzymatic synthesis of d-pantothenic acid

An asymmetric sustainable route to d -pantothenic acid was developed, centering on the reduction of ethyl 2′-ketopantothenate to ethyl ( R )-pantothenate, enabled by an E. coli strain co-expressing an engineered ketoreductase and glucose dehydrogenase.