微泡
核糖核酸
小RNA
小核仁RNA
生物
非编码RNA
信使核糖核酸
生物发生
作者
Kurt Januszyk,Eva-Maria Weick,Christopher D. Lima
出处
期刊:Methods of Molecular Biology
日期:2020-01-01
卷期号:2062: 467-489
标识
DOI:10.1007/978-1-4939-9822-7_23
摘要
We describe procedures to clone, express, and reconstitute an active human nuclear RNA exosome. Individual recombinant subunits are expressed from E. coli and successfully reconstituted into the nuclear complex, which contains the noncatalytic nine-subunit exosome core, the endoribonuclease and exoribonuclease DIS3, the distributive exoribonuclease EXOSC10, the cofactors C1D and MPP6, and the RNA helicase MTR4.
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