Three new steroidal components from the roots of reineckia carnea

A new coprostanol glycoside, 26-trihydroxy-16, 22-dioxo-3β-[(α-L-rhamnopyranosyl)oxy]-5β-cholestan-1β-yl O-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside (1) (25S)-5β-spirostan-1β,2β,3β, 4β,5β, 6β-hexol (2), a new C-22 steroidal lactone saponin, (20 R)-16β-trihydroxy-3β-[(α-L-rhamnopyranosyl)oxy]-1β-[(O-α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranosyl)oxy]-5β-pregnane-20-oic acid γ-lactone (3) along with two known pregnane glycosides (4 and 5) were obtained from the roots of Reineckia carnea. Their structures were determined by 1 D, 2 D NMR, IR and MS data analysis. In addition, the cytotoxic activities in HT-29, HCT116, H1299 and A549 tumor cells of the isolated compounds (1 − 5) were determined by the MTT method. The results showed that only compound 1 exhibited weak effect against these cells with IC50 values ranging from 63.36 μM to 105.01 μM.