The damage on human umbilical vein endothelial cell lines induced by trichlorfon

Objective To explore the time-effect of damage in human umbilical vein endothelial cell lines induced by Trichlorfon and to analyze its mechanism.Methods HUVECs-12 were cultured in DMEM containing Trichlorfon for 24 h,the growth inhibition was measured by MTT assay.Cells were incubated in IC50 concentration Trichlorfon(400 μmol/L) for different times(0,4,6,8,12,24,48 h)) to test the time-effect of damage in human umbilical vein endothelial cells;the level of malondialde-hyde(MDA) in supernatant-conditioned media was monitored by thiobituric acid reaction(TBA).Results Trichlorfon inhibited HUVECs-12 growth in a dose-dependent manner and increased the production of MDA.Conclusion Trichlorfon can induce damage in human umbilical vein endothelial cells.