生物
翻译(生物学)
细胞生物学
蛋白质生物合成
平动调节
核糖核酸
信使核糖核酸
核糖体
非翻译区
平移移码
EIF4E公司
核糖体分析
真核翻译
计算生物学
遗传学
基因
作者
Xiao‐Wei Yan,Tim A. Hoek,Ronald D. Vale,Marvin E. Tanenbaum
出处
期刊:Cell
[Elsevier]
日期:2016-05-01
卷期号:165 (4): 976-989
被引量:386
标识
DOI:10.1016/j.cell.2016.04.034
摘要
Regulation of mRNA translation, the process by which ribosomes decode mRNAs into polypeptides, is used to tune cellular protein levels. Currently, methods for observing the complete process of translation from single mRNAs in vivo are unavailable. Here, we report the long-term (>1 hr) imaging of single mRNAs undergoing hundreds of rounds of translation in live cells, enabling quantitative measurements of ribosome initiation, elongation, and stalling. This approach reveals a surprising heterogeneity in the translation of individual mRNAs within the same cell, including rapid and reversible transitions between a translating and non-translating state. Applying this method to the cell-cycle gene Emi1, we find strong overall repression of translation initiation by specific 5′ UTR sequences, but individual mRNA molecules in the same cell can exhibit dramatically different translational efficiencies. The ability to observe translation of single mRNA molecules in live cells provides a powerful tool to study translation regulation.
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