Implementation of Measurement System of Concentration of Ca~(2+) Based on Microfluidic Chip

Microfluidic chip has been introduced to the quantitative analysis of materials within cells.Biotic experiments such as cell fostering,staining,and injecting reagents can be implemented on the designed microfluidic chip.This paper presents a microfluidic fluorescence detection system to measure the concentration of Ca2+ in cells.By dual wavelength light excite,the fluorescence signal and image respectively were captured by fluorescence detection system.The concentration of Ca2+ in cells can be calculated by using the fluorescence ratio method.The experimental results confirm that the system is stable and credible.Research indicates a new cell detection means.A convenient and swift detecting device of cells fostering,cells detecting,and injecting reagents were provided to the research of cells.