Immunogenicity ofPasteurella multocidaGrown in Iron-restricted Medium

Abstract Srivastava, S.K. 1998. Imrnunogenicity of Pasteu rella multocida grown in iron restricted medium. J. Appl. Anim. Res., 13: 137–144. Pasteurella multocida semtype B:2 was gmwn under imn-restriction condition to enhance the growth of outer membrane protein (OMP) and tested for immunogenicity. Sodium dodecyl sulphate polyacrylamide gel electmphresis revealed enhanced production of an 84 KDa protein but decrease in pmduction of a pmtein with a molecular weight of 16 KDa. No difference in the quantity of OMP produced by the cells grown under iron- restriction and iron-sufficient conditions was observed as deduced by Congo red binding assay. Vaccines prepared fmm P. multocida cells gmwn in these 2 conditions did not differ from each other in conferring protection in mice and mbbits. However, the antibody titres detected in rabbits were significantly higher with the vaccine consisting of P. multocida cells gmwn under restriction than those grown under iron-sufficient conditions. Similar results were also observed in cattle suggesting an improved immunogenicity of the former. It was concluded that a vawine consisting of P. multocida cells grown under imn-restriction could be more effective in animals than the vaccine prepared from cells gmwn conventionally.